ABSTRACT
Objective:To measure the parameters of median nerve under ultrasound in patients with diabetes mellitus to explore the relationship between ultrasonic parameters and nerve conduction study. Methods:From June to November, 2019, 79 diabetic patients were divided into diabetic peripheral neuropathy (DPN) group (n = 37) and non-DPN group (n = 42) according to the diagnosis. Their right median nerve were measured cross-sectional area (CSA) and the proportion of hypoechoic areas (HA) 5 cm and 10 cm proximal to the wrist (CSA-5, CSA-10, HA-5 and HA-10, respectively) with sonography; while the conductive latency and velocity of right median nerves were measured with nerve conductive study. Results:CSA-5, CSA-10 and HA-10 were more in DPN group than in non-DPN group (|Z| > 2.282, P < 0.05). HA-5 correlated with the motor conductive latency (r = 0.264, P < 0.05) and velocity (r = -0.270, P < 0.05); HA-10 correlated with the abnormal sensory conductive latency (r = 0.234, P < 0.05) and velocity (r = 0.363, P < 0.01), and the motor conductive latency (r = 0.235, P < 0.05) and velocity (r = -0.255, P < 0.05). Conclusion:DPN can be found under sonography, some of them are related with the nerve electrophysiology, which may be a useful tool to screen DPN.
ABSTRACT
Based on the complete genome sequence of pigeon-origin Newcastle disease virus strain JS/07/04/ Pi(genotype VIb), nine overlapped fragments covering its full-length genome were amplified by RT-PCR. The fragments were connected sequentially and then inserted into the transcription vector TVT7/R resulting in the TVT/071204 which contained the full genome of strain JS/07/04/Pi. The TVT/071204 was co-transfected with three helper plasmids pCI-NP, pCI-P and pCI-L into the BSR cells, and the transfected cells and culture supernatant were inoculated into 9-day-old SPF embryonated eggs 60 h post-transfection. The HA and HI tests were conducted following the death of embryonated eggs. The results showed that the allantoic fluids obtained were HA positive and the HA could be inhibited by anti-NDV serum which indicated that the strain JS/07/04/Pi was rescued successfully. The rescued virus rNDV/071204 showed similar growth kinetics to its parental virus in CEF. The successful recovery of this strain would contribute to the understanding of the host-specificity of pigeon-origin NDV and to the development of the novel vaccines against the NDV infection in pigeons.